Predoctoral research fellowship in AAV9-mediated gene therapy

Overview

Prof. Alessandra Bolino is seeking a highly motivated research fellow to join the research team at the San Raffaele Scientific Institute in Milan, Italy (

This role is central to an innovative research program dedicated to developing advanced therapeutic strategies for the most severe forms of inherited peripheral neuropathies with childhood onset, the demyelinating Charcot-Marie Tooth type 4.

The successful candidate will test the efficacy of AAV9-mediated gene replacement strategies using systemic and intrathecal delivery. Taking advantage of extensively characterized in vivo and ex vivo disease models, this project sits at the forefront of genetic medicine, utilizing viral vector technology to address currently unmet medical needs in pediatric neurology.

This position provides direct collaboration with world-leading investigators in the field of neuromuscular disorders and gene therapy across both Europe and the United States.

Qualifications

• Applicants must hold a degree in Medicine (MD) or Pharmaceutical Chemistry and Technology (CTF), or a Master’s degree in Biotechnology or Biology;
• Applicants possess demonstrated interest in neuroscience, supported by a strong academic record and hands-on laboratory training;
• Independent research capability;
• Organizational skills;
• A primary requirement is a demonstrated commitment to pursuing advanced academic research through a PhD program.

Responsibilities

• Molecular cloning of AAV9 vectors specifically tailored for gene replacement studies;
• In vivo assessments in established murine models of inherited neuropathies;
• Conduct comprehensive multi-parametric analyses, including:
• Molecular: Quantitative PCR (DNA/RNA) and RNAscope technology.
• Morphological: Immunohistochemistry and advanced histology.
• Functional: Neurophysiological and biochemical evaluations.
• Ensure rigorous data integrity via Electronic Lab Notebooks (ELN).

How To Apply

Please submit a letter of intent, your curriculum vitae, and the contact information for academic references to Alessandra Bolino,

At San Raffaele Scientific Institute, a world-renowned center of excellence for clinical and scientific activities, we believe that embracing diversity and promoting an open and inclusive environment are crucial to our success.

We are committed to ensuring that equal employment opportunities are provided based on merit, without regard to age, religion, sex, disability, medical condition, sexual orientation, cultural or social background, gender identity or expression, national or ethnic origin, or any other legally recognized status protected under applicable laws.

We offer attractive conditions and rewards appropriate for an internationally competitive research organization.